Mouse Submandibular Gland
Mouse Submandibular Gland - Related Videos
05:18
Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct
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Cited by 17 •
2011
A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.
07:10
In Vivo Vascular Permeability Detection in Mouse Submandibular Gland
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Cited by 2 •
2022
In the present protocol, the endothelial barrier function of the submandibular gland (SMG) was evaluated by injecting different molecular weighted fluorescent tracers into the angular veins of test animal models in vivo under a two-photon laser-scanning microscope.
07:44
Retroductal Submandibular Gland Instillation and Localized Fractionated Irradiation in a Rat Model of Salivary Hypofunction
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Cited by 5 •
2016
Salivary gland hypofunction, a major adverse effect of head and neck radiotherapy diminishes a patient's quality of life. The demonstration of efficacy of new therapies in animal models is a prerequisite before clinical transition. This protocol describes retroductal administration and local irradiation of rat submandibular glands.
07:45
Retroductal Nanoparticle Injection to the Murine Submandibular Gland
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Cited by 10 •
2018
Local drug delivery to the submandibular glands is of interest in understanding salivary gland biology and for the development of novel therapeutics. We present an updated and detailed retroductal injection protocol, designed to improve delivery accuracy and experimental reproducibility. The application presented herein is the delivery of polymeric nanoparticles.
09:52
Isolation of Mouse Salivary Gland Stem Cells
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Cited by 41 •
2011
An optimized protocol for the isolation of stem cells from the mouse salivary gland is described. The method employs enzymatic and mechanical digestion, and permits isolation of salispheres containing cells with characteristics of stem cells.
FAQs
Acinar cells initiate secretion by releasing fluid, electrolytes, and proteins, while ductal cells subsequently modify that material before it exits the gland. This division of labor allows researchers to distinguish defects in initial secretory activity from abnormalities in ductal processing, helping clarify how glandular function changes during injury, disease, or experimental treatment.
Autonomic nerve signals adjust secretion according to physiological demands, linking gland activity to the organism’s changing needs. This regulatory relationship gives investigators a way to study how neural control affects exocrine output and how disruptions in signaling may alter gland performance. It also provides context for evaluating treatments that influence secretory regulation.
Drugs, hormones, and genetic changes can be evaluated for their effects on exocrine secretion and gland maintenance. Because the gland has defined secretory and ductal cell populations, experiments can relate an intervention to altered saliva production, modified gland support, or impaired tissue upkeep. These comparisons help identify how specific factors influence normal or diseased gland behavior.
Studies can examine glandular function, exocrine secretion, tissue maintenance, and responses to injury or inflammation. Researchers can also investigate developmental changes, regenerative capacity, and cancer-related alterations. Considering these outcomes together is useful because a treatment or genetic change may affect both immediate secretory performance and the longer-term ability of the gland to remain healthy.
Its accessible anatomy and well-characterized cellular organization make the mouse submandibular gland a practical model for examining development, tissue injury, regeneration, inflammation, and cancer. Researchers can connect visible or functional changes with defined glandular structures, allowing the same organ system to support investigations of normal biology, repair processes, and disease-related mechanisms.
In medicine, the model supports controlled investigation of salivary gland maintenance and disease processes while allowing researchers to test how drugs, hormones, or genetic changes influence the tissue. Findings can be organized around secretion, injury responses, regeneration, inflammation, or cancer, making the gland relevant to both basic studies and disease-focused experimental research.