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In Vivo Vascular Permeability Detection in Mouse Submandibular Gland
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In Vivo Vascular Permeability Detection in Mouse Submandibular Gland

Article DOI: 10.3791/64167-v • 07:10 min • August 4th, 2022
August 4th, 2022 •
1, 2, 3, 1
1Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research, 2Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Reseah Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, 3State Key Laboratory of Natural and Biomimetic Drugs, Peking University

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Summary
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In the present protocol, the endothelial barrier function of the submandibular gland (SMG) was evaluated by injecting different molecular weighted fluorescent tracers into the angular veins of test animal models in vivo under a two-photon laser-scanning microscope.

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In Vivo Vascular Permeability Detection Mouse Submandibular Gland Tight Endothelial Junctions Two Photon Laser Scanning Microscopy Confocal Microscopy Tracers Fluorescein Isothiocyanate Labeled Dextran Rhodamine B Phosphate Buffered Saline Permeability Assay Male Wild Type Mouse Anesthetizing Eyeball Insulin Syringe Fluorescent Tracer Solution Mixture Supine Position Submandibular Gland Isolation Stereoscopic Micro
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