Murine Cardiac Imaging
Murine Cardiac Imaging - Related Videos
11:37
Cardiac Magnetic Resonance Imaging
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2023
Source: Frederick W. Damen and Craig J. Goergen, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana In this video, high field, small-bore magnetic resonance imaging (MRI) with physiological monitoring is demonstrated to acquire gated cine loops of the murine cardiovascular system. This procedure provides a basis for assessing left-ventricular function, visualizing vascular networks, and quantifying motion of organs due to respiration. Comparable small animal...
09:14
Cardiac Magnetic Resonance Imaging at 7 Tesla
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Cited by 10 •
2019
The sensitivity gain inherent to ultrahigh field magnetic resonance holds promise for high spatial resolution imaging of the heart. Here, we describe a protocol customized for functional cardiovascular magnetic resonance (CMR) at 7 Tesla using an advanced multi-channel radio-frequency coil, magnetic field shimming and a triggering concept.
09:00
Murine Echocardiography and Ultrasound Imaging
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Cited by 61 •
2010
This video demonstrates use of a rail-mounted high-frequency ultrasound probe to perform echocardiography on an anesthetized mouse. The methods describe both conventional two-dimensional and M-mode measurements of cardiac function in addition to newer, more powerful tools such as color Doppler, strain analysis, as well as general and targeted contrast imaging.
03:02
Immunostaining of Bacteria-Filled Microlesions in a Mouse Cardiac Tissue Section for Imaging
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2026
Source: Brown, A. O., and Orihuela, C. J. Visualization of Streptococcus pneumoniae within Cardiac Microlesions and Subsequent Cardiac Remodeling. J. Vis. Exp. (2015)The video demonstrates the immunostaining of chemically fixed mouse cardiac tissue sections to visualize pathogenic bacteria localized within infection-induced microlesions. The process includes tissue washing, membrane permeabilization, blocking of nonspecific sites, antibody labeling, nuclear counterstaining, and confocal...
08:52
Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection
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Cited by 13 •
2015
This protocol describes how to produce functional sinus nodal tissue from murine pluripotent stem cells (PSC). T-Box3 (TBX3) overexpression plus cardiac Myosin-heavy-chain (Myh6) promoter antibiotic selection leads to highly pure pacemaker cell aggregates. These “Induced-sinoatrial-bodies” (“iSABs”) contain over 80% pacemaker cells, show highly increased beating rates and are able to pace myocardium ex vivo.
FAQs
Each modality converts a different physical signal into a distinct cardiac measurement. Echocardiography uses ultrasound reflections to characterize ventricular dimensions and contraction, whereas magnetic resonance imaging derives information from magnetic signals. Computed tomography relies on X-ray attenuation, and positron emission tomography maps radiotracer distribution. This separation lets investigators match structural, functional, perfusion, or metabolic questions to appropriate measurements.
The relevant endpoint determines the most informative modality. Ventricular dimensions and myocardial contraction call for measurements of anatomy and motion, while perfusion, metabolism, or blood flow require readouts suited to circulation or tissue activity. This endpoint-driven choice helps relate observed cardiac changes to disease processes or treatment responses in preclinical studies.
Repeated imaging follows the same animal across time, allowing investigators to evaluate progression and treatment response while preserving physiological context. This design can require fewer animals and reduces dependence on comparisons between separate groups at each time point. It therefore supports observation of evolving cardiac outcomes rather than a single snapshot.
Imaging supplies whole-organ outcomes that can be interpreted alongside molecular and cellular findings. A study can examine whether those lower-level observations correspond to altered ventricular dimensions, contraction, perfusion, metabolism, or remodeling. This connection places mechanistic discoveries within the intact heart and relates them to cardiovascular disease and treatment responses in preclinical medicine.
Investigators first identify whether the study needs information about structure, contraction, blood flow, perfusion, metabolism, or remodeling. They then select an imaging approach whose detected signal can provide that measurement, collect data in living animals, and compare results over time or after treatment. The workflow preserves links between an intervention and its cardiac outcome.
These methods are useful for studying cardiovascular disease and evaluating responses to treatment in living animals. Depending on the selected approach, investigators can monitor ventricular changes, myocardial contraction, perfusion, metabolism, or remodeling. Such measurements provide preclinical evidence about how disease or an intervention affects the heart as a whole, rather than only its molecular or cellular components.