Serum Isolation
Serum Isolation - Related Videos
06:44
Isolation of Small Noncoding RNAs from Human Serum
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Cited by 13 •
2014
This protocol describes a method for extracting small RNAs from human serum. We have used this method to isolate microRNAs from cancer serum for use in DNA arrays and also singleplex quantitative PCR. The protocol utilizes phenol and guanidinium thiocyanate reagents with modifications to yield high quality RNA.
08:59
Isolation and Culture of Microglia from Rat Brains using Serum-Free Media
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2025
This video demonstrates a method for isolating and culturing microglia from rat brains. It entails separating microglia from other neuronal and non-neuronal cells using plates coated with a monoclonal antibody specific to microglia.
08:43
Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
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Cited by 9 •
2017
Endothelial progenitor cells (EPCs) are crucially involved in the neovascularization of ischemic tissues. This method describes the isolation of human EPCs from peripheral blood, as well as the identification of their migratory potential against serum samples of cardiac surgical patients.
12:00
Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium
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Cited by 26 •
2018
Efforts to understand microglial function in detail have been hindered by the lack of microglial culture models that recapitulate the properties of mature in vivo microglia. This protocol describes an isolation and culture approach designed to maintain robust survival of highly ramified mature rat microglia under defined-medium conditions.
02:52
Development and Maintenance of Serum-Free Embryoid Bodies
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2025
The video describes the procedure for developing serum-free embryoid bodies (SFEBs) from neuronal progenitor cells using various serum-free differentiation mediums or DM. DM-2 facilitates neurons' maturation and arrangement to form SFEBs, while DM-3 is used for further growth maintenance.
FAQs
Clot formation removes clotting components from the fraction intended for serum analysis. Once whole blood has clotted, centrifugation can compact the clot and cellular material, leaving a clearer liquid portion for recovery. This separation matters because the resulting sample is suited to assays that measure soluble proteins, antibodies, hormones, metabolites, and other biomarkers.
Centrifugation separates the liquid serum from the compact clot and cellular material produced during clotting. The process concentrates the unwanted solid components so the clearer liquid fraction can be collected for testing. Consistent centrifugation is therefore central to obtaining a serum sample whose composition supports reliable medical analyses and biochemical assays.
Hemolysis and contamination are handling concerns because they can reduce the reliability of results obtained from serum. Careful collection and processing help preserve the intended liquid sample and limit unwanted material. This attention to sample quality is especially important when serum is used to evaluate soluble biomarkers or compare findings across medical or research analyses.
A basic workflow begins by collecting whole blood and allowing it to clot. The clotted sample is then centrifuged to separate the clear liquid from the clot and cellular material. Finally, the serum fraction is carefully recovered for analysis. Controlled handling throughout these steps helps minimize hemolysis and contamination and supports consistent results.
Serum provides access to a broad range of soluble biological components, including proteins, antibodies, hormones, metabolites, and other biomarkers. Their measurement can support diagnostic testing, disease monitoring, immunological studies, therapeutic research, and biochemical assays. The specific information obtained depends on the analytical test applied to the isolated sample.
Serum isolation is useful when investigators need a liquid blood-derived sample for diagnostic testing, monitoring disease-related changes, or studying immune responses. It also supports therapeutic research and biochemical assays. Because these applications depend on measurable soluble components, consistent collection and processing are important for producing results that can be interpreted confidently.