Click Chemistry
Click Chemistry - Related Videos
08:42
Coordination Chemistry Complexes
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2023
Source: Laboratory of Dr. Neal Abrams — SUNY College of Environmental Science and Forestry Transition metals are found everywhere from vitamin supplements to electroplating baths. Transition metals also make up the pigments in many paints and compose all minerals. Typically, transition metals are found in the cationic form since they readily oxidize, or lose electrons, and are surrounded by electron donors called ligands. These ligands do not form ionic or covalent bonds with the metal center,...
12:31
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
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Cited by 14 •
2012
Adenovirus particles are engineered to contain either the unnatural amino acid analogue azidohomoalanine or the azido sugar O-GlcNAz. The azide group of each is chemoselectively ligated via "click" chemistry reactions as a means of viral surface modification.
09:14
Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
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Cited by 8 •
2016
This protocol details the important steps required for the bioconjugation of a cysteine containing protein to a maleimide, including reagent purification, reaction conditions, bioconjugate purification and bioconjugate characterization.
03:08
Basic Organic Chemistry Techniques - Concepts
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2020
Glassware in Organic Chemistry There are standard pieces of glassware that are used in the organic chemistry lab. Beakers and Erlenmeyer flasks are typically used for simple mixing or to hold solvents but they should not be used to measure volume, unless only an approximate volume is needed. Glassware can be divided into the following categories: Volumetric Glassware – This includes glassware such as graduated cylinders, volumetric glass pipettes, and volumetric flasks. The purpose of these...
02:17
What is Organic Chemistry?
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2023
Organic chemistry is the study of compounds of carbon called organic compounds. Organic compounds either originate from living organisms or are synthesized by chemists. A defining trait of these compounds is the presence of carbon as the principal element, which is bonded to other carbon atoms and other elements such as hydrogen, oxygen, nitrogen, and sulfur. The existence of a wide array of organic molecules is a consequence of carbon atoms’ ability to form up to four strong bonds to other...
FAQs
The two major routes differ in how the reactive partners are activated. Copper-catalyzed azide-alkyne cycloaddition uses copper to promote coupling, whereas strain-promoted cycloaddition uses a strained alkyne and avoids copper. This distinction lets investigators choose a labeling strategy that fits the biological setting, especially when introducing or excluding a metal catalyst matters.
Selectivity comes from pairing complementary functional groups that react with one another more readily than with surrounding biomolecules. Because the reaction can proceed with limited interference from those biomolecules, a fluorescent tag, affinity handle, or probe can be directed to a chosen molecular target. This chemical discrimination is central to resolving molecular events in complex neural samples.
Aqueous compatibility allows these reactions to be carried out in conditions relevant to biological research rather than requiring a chemically isolated environment. That feature helps preserve the usefulness of labeling strategies for neuronal proteins, glycans, lipids, and other targets. Limited interference from surrounding biomolecules further supports selective analysis within complex neural material.
A typical workflow starts by selecting a target and introducing, or identifying, the complementary functional group needed for the reaction. The researcher then chooses either a copper-catalyzed or copper-free coupling route, supplies the desired fluorescent tag, affinity handle, or probe, and performs the reaction under compatible conditions. The resulting attachment supports downstream molecular analysis.
In neuroscience, researchers can use these reactions to attach fluorescent tags for imaging, affinity handles for molecular capture, or probes for tracking. The targets may include neuronal proteins, glycans, and lipids. These applications help examine where molecules are located, how they move or are organized, and how molecular components relate to synaptic organization and neural signaling.
The attached chemical reporter determines the type of information available. Fluorescent tags support visualization, affinity handles support isolation or molecular capture, and probes support molecular tracking. Together, these outputs can connect the presence or movement of neuronal molecules with synaptic organization and neural signaling, extending analysis beyond simply identifying a biochemical target.