Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

The overall goal of synthesizing protein bioconjugates via cysteine-maleimide chemistry is to ensure specificity in the site of modification on the protein. Which in turn effects the activity of the resulting bioconjugate, for example, antibody drug conjugates. If you're interested in protein structure or function and you do research in nano-medicine, fluorescent microscopy, or systems chemistry, then this technique will help you answering your questions.

The main advantage of this technique is that it is highly specific and high yielding under more conditions. To begin this procedure, dissolve 12 milligrams of lyophilized cytochrome C in six milliliters of 20 milimolar phosphate buffer. Pipette 16 microliters of freshly prepared one molar DTT solution into the protein solution to reduce the cytochrome C.After mixing the solution, filter it through a 0.22 micron low protein binding PBDF syringe filter before injecting onto any chromotographic media.