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Immunology and Infection
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Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
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Immunology and Infection
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JoVE Journal Immunology and Infection
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

DOI:
10.3791/4246-v

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12:31 min

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August 19, 2012

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, , , ,
1Department of Chemistry,Stony Brook University

Chapters

  • 00:00Title
  • 02:04Production of Aha-Labeled Adenovirus
  • 04:28Production of Azido-Sugar-Labeled Adenovirus
  • 05:46Purification of Azide-Labeled Adenoviral Particles
  • 07:34Ligation via Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC)
  • 08:32Ligation via Copper(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC)
  • 09:32SDS-PAGE Analysis of Clicked Adenovirus
  • 10:34Conclusion

Summary

Automatic Translation

Automatic Translation

Adenovirus particles are engineered to contain either the unnatural amino acid analogue azidohomoalanine or the azido sugar O-GlcNAz. The azide group of each is chemoselectively ligated via "click" chemistry reactions as a means of viral surface modification.

Tags

Chemoselective ModificationViral SurfacesBioorthogonal Click ChemistryGene TherapyOncolytic ApplicationsVaccine DevelopmentGenetics-based ApproachesChemoselective LigationTargeting MoleculesDyesAzide TagsMetabolic LabelingEffector LigandsAdenovirus ParticlesAzidohomoalanineO-linked N-azidoacetylglucosamine

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