Cutting Floating Method
Cutting Floating Method - Related Videos
05:00
The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning
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Cited by 21 •
2018
Here, we present a protocol to improve paraffin sectioning. This method combines cutting and floating using a simple thermostatic chamber to avoid the transfer process required by the conventional method. As a result, the efficiency and the number of intact paraffin sections were greatly improved.
13:10
Stability of Floating Vessels
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2023
Source: Alexander S Rattner and Kevin Rao Li Department of Mechanical and Nuclear Engineering, The Pennsylvania State University, University Park, PA The objective of this experiment is to demonstrate the phenomenon of stability of floating vessels - the ability to self-right when rolled over to the side by some external force. Careful design of hull shapes and internal mass distribution enables seagoing vessels to be stable with low drafts (submerged depth of hull), improving vessel...
09:38
Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples
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Cited by 22 •
2016
This protocol describes a simple Hybrid-Cut tissue sectioning method that is useful for recalcitrant plant tissues. Good quality tissue sections enable anatomical studies and other biological studies including in situ hybridization (ISH).
07:58
Free-floating Immunostaining of Mouse Brains
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Cited by 10 •
2021
This protocol describes an efficient and reproducible approach for mouse brain histological studies, including perfusion, brain sectioning, free-floating immunostaining, tissue mounting, and imaging.
10:11
Collection and Long-Term Maintenance of Leaf-Cutting Ants (Atta) in Laboratory Conditions
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Cited by 6 •
2022
Here, a protocol is described to successfully collect and maintain healthy Atta (Hymenoptera: Formicidae) ant colonies in laboratory conditions. Additionally, different nest types and configurations are detailed together with possible experimental procedures.
FAQs
Suspending each section prevents it from depending on contact with a slide or other surface during staining. Reagents can reach exposed tissue more evenly, which is particularly valuable when several incubation and washing steps are required. This arrangement can therefore support more consistent labeling throughout a section and preserve access to organized neural structures.
The buffered solution provides the immersion environment during sectioning, allowing each newly produced slice to remain suspended rather than adhere immediately to a support. That separation helps maintain the free-floating format used for subsequent reagent exposure. The approach is therefore suited to protocols in which tissue must remain accessible from multiple sides.
Its main procedural distinction is that sections remain suspended in solution instead of being fixed to a slide during the processing sequence. This changes how reagents contact the tissue and makes the slices easier to handle through multi-step staining workflows. Surface-independent processing is especially relevant when preserving access across the full section matters.
A fixed brain or neural tissue block is placed in a buffered solution and sectioned while immersed. The resulting slices are kept suspended as free-floating sections, then made available for reagent-based processing and microscopic examination. This workflow connects tissue preparation directly with staining procedures such as immunohistochemistry and with later structural analysis.
They are particularly useful when immunohistochemistry requires multiple processing stages, because suspended tissue remains accessible during repeated reagent exposure. The method can support labeling of proteins and cell types across the section, allowing investigators to examine where specific signals occur within organized neural tissue. These observations can contribute to anatomical mapping and microscopic interpretation.
Serial free-floating slices provide consecutive views through a tissue block. Examining those sections together allows researchers to follow structures across multiple levels rather than interpreting a single plane alone. In neuroscience, this supports three-dimensional examination of neural organization and can help map proteins, cell types, neuronal morphology, or pathways across related tissue slices.