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Free-floating Immunostaining of Mouse Brains

Free-floating Immunostaining of Mouse Brains

Article DOI: 10.3791/62876-v 07:58 min October 7th, 2021
October 7th, 2021



This protocol describes an efficient and reproducible approach for mouse brain histological studies, including perfusion, brain sectioning, free-floating immunostaining, tissue mounting, and imaging.



Immunostaining Mouse Brains Neuroscience Research Histology Reproducibility Reliability Methodology Histological Studies Anesthetization C57 Black/6J Mouse Foam Board Incision Muscle Wall Thorax Abdomen Ribcage Perfusion Cannula Heart Ventricle Ascending Aorta Pins Saline Pressure Switch
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