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Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples
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Biology
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JoVE Journal Biology
Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples

Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples

DOI:
10.3791/54754-v

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09:38 min

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November 23, 2016

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, , , , ,
1Academia Sinica Biotechnology Center in Southern Taiwan; Agricultural Biotechnology Research Center,Academia Sinica

Chapters

  • 00:05Title
  • 01:10Plant Tissue Collection and Preparation
  • 03:35Tissue Embedding and Hybrid-Cut Sectioning
  • 05:24Tissue Staining
  • 06:54Results: Hybrid-Cut Sectioning Allows for Sectioning of Recalcitrant Plant Tissues, and Improves Tissue Integrity for Staining and In Situ Hybridization
  • 08:32Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a simple Hybrid-Cut tissue sectioning method that is useful for recalcitrant plant tissues. Good quality tissue sections enable anatomical studies and other biological studies including in situ hybridization (ISH).

Tags

Keywords: Hybrid-cutParaffinCryostatIn Situ HybridizationFISHPhalaenopsis OrchidFixationDehydrationInfiltrationParaffin Embedding

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