Gfp Reporter Mice
Gfp Reporter Mice - Related Videos
06:19
In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy
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Cited by 5 •
2017
This protocol describes how high-resolution imaging techniques such as spectral domain optical coherence tomography and scanning laser ophthalmoscopy can be utilized in small rodents, using an ophthalmic imaging platform system, to obtain information on retinal thickness and microglial cell distribution, respectively.
03:38
Measuring Mycobacterium smegmatis Mistranslation Rates Using a GFP/Luciferase Reporter System
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2025
Source: Chen, Y., et al., Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems. J. Vis. Exp. (2019)This video demonstrates a method to quantify mistranslation in Mycobacterium smegmatis using an inducible GFP and secreted mutant luciferase reporter system. It shows how luminescence resulting from mistranslation is measured and compared to GFP fluorescence to evaluate the impact of a test molecule on translational accuracy.
09:22
Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
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Cited by 11 •
2013
This protocol describes an experimental procedure for the rapid construction of artificial transcription factors (ATFs) with cognate GFP reporters and quantification of the ATFs ability to stimulate GFP expression via flow cytometry.
11:44
Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice
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Cited by 27 •
2015
Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.
10:40
Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo and In Vitro
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2025
The protocol uses an adipocyte-specific CD63-GFP reporter mouse line to visualize and quantify the secretion of adipocyte-derived extracellular vesicles (EVs) and demonstrate their uptake by progenitor cells, revealing a paracrine pathway within adipose tissue.
FAQs
The promoter or regulatory sequence linked to GFP determines which genetic program controls fluorescence. When that program is active, the corresponding cells produce the reporter signal, allowing researchers to associate fluorescence with a particular cellular state or population. This design is central to interpreting labeled neurons and other cells in neuroscience experiments.
Fluorescence provides more than a visible marker of where cells are located. Because GFP expression follows a selected regulatory sequence, the signal can indicate where that genetic program is active within tissue. Researchers can therefore examine cellular organization while relating the observed pattern to gene expression in developing or mature neural populations.
The tissue context and experimental goal guide the imaging choice. GFP can be detected in living preparations for longitudinal imaging, allowing labeled cells to be followed over time, or in preserved tissue for detailed examination after collection. These complementary approaches support both dynamic observations and structural analysis of neural organization.
Fluorescent labeling makes selected cells recognizable during analysis and can support targeted cell isolation. After isolating the GFP-positive population, researchers can examine that defined group rather than treating all cells in a sample as equivalent. In neuroscience, this helps connect cellular identity or organization with properties of particular neuronal populations.
Researchers can track fluorescently labeled populations as neural structures form and become organized. The resulting patterns help map where selected cells are located and how they are distributed within neural tissue. This approach provides a way to examine developmental changes alongside the arrangement of cells that contributes to circuit organization.
Comparing fluorescence patterns or labeled-cell characteristics before and after injury or disease can reveal changes in the affected population. The model helps researchers determine whether labeled cells alter their distribution or organization under these conditions. Such observations provide cellular context for studying how pathological or injury-related processes affect neural tissue.