Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

Felix Heymann; Patricia M. Niemietz; Julia Peusquens; Can Ergen; Marlene Kohlhepp; Jana C. Mossanen; Carlo Schneider; Michael Vogt; Rene H. Tolba; Christian Trautwein; Christian Martin; Frank Tacke

doi:10.3791/52607

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Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

11,912 Views

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11:44 min

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March 24, 2015

DOI:

10.3791/52607-v

DOI:

10.3791/52607-v

•

11:44 min

March 24, 2015

•

6 Views

Felix Heymann*¹, Patricia M. Niemietz*¹, Julia Peusquens¹, Can Ergen¹, Marlene Kohlhepp¹, Jana C. Mossanen¹, Carlo Schneider¹, Michael Vogt², Rene H. Tolba³, Christian Trautwein¹, Christian Martin⁴, Frank Tacke¹

¹Department of Medicine III,RWTH University-Hospital Aachen, ²IZKF Aachen Core Facility "Two-Photon Imaging",RWTH University-Hospital Aachen, ³Institute for Laboratory Animal Science & Experimental Surgery,RWTH Aachen University, ⁴Institute for Pharmacology,RWTH University-Hospital Aachen

Summary

Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.