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Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

DOI:
10.3791/52607-v

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11:44 min

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March 24, 2015

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, , , , , , , , , , ,
1Department of Medicine III,RWTH University-Hospital Aachen, 2IZKF Aachen Core Facility "Two-Photon Imaging",RWTH University-Hospital Aachen, 3Institute for Laboratory Animal Science & Experimental Surgery,RWTH Aachen University, 4Institute for Pharmacology,RWTH University-Hospital Aachen

Chapters

  • 00:05Title
  • 02:50Tracheotomy
  • 04:36Laparatomy
  • 05:50Sample Setup
  • 08:39Results: Intravital TPLSM with CCI4 Damaged Livers
  • 10:02Conclusion

Summary

Automatic Translation

Automatic Translation

Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.

Tags

Keywords: Intravital MicroscopyMultiphoton ImagingLiver InflammationImmune CellsCXCR6.Gfp Reporter MiceLeukocyte MigrationLiver InjuryCarbon Tetrachloride

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