Hippocampus Electroporation
Hippocampus Electroporation - Related Videos
06:30
Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation
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Cited by 27 •
2012
This article describes in detail a protocol to electroporate in utero the cerebral cortex and the hippocampus at E14.5 in mice. We also show that this is a valuable method to study dendrites and spines in these two cerebral regions.
06:00
Cortex-, Hippocampus-, Thalamus-, Hypothalamus-, Lateral Septal Nucleus- and Striatum-specific In Utero Electroporation in the C57BL/6 Mouse
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Cited by 14 •
2016
This protocol describes in detail how to specifically transfect different regions in the C57BL/6 central nervous system via in utero electroporation. Included in this protocol are detailed instructions for transfections of regions that develop into the cortex, hippocampus, thalamus, hypothalamus, lateral septal nucleus and striatum.
08:05
Murine In Utero Electroporation
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2023
In utero electroporation is an important technique for studying the molecular mechanisms that guide the proliferation, differentiation, migration, and maturation of cells during neural development. Electroporation enables the rapid and targeted delivery of material into cells by utilizing electrical pulses to create transient pores in cell membranes. Although electroporation has traditionally been used in in vitro studies, scientific advancements have now broadened its utilization to intact...
11:57
Electroporation of Mycobacteria
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Cited by 55 •
2008
Mycobacterial pathogenic strategies remain poorly understood. The slow growth rate of most species, the impenetrable nature of the cell-wall, and the hazards of working with pathogens make mycobacteria difficult to study and are largely responsible for our poor understanding of these organisms. In this video we will demonstrate the technique of electroporation, which involves subjecting cells to a brief high electrical impulse to allow the entry of DNA. It is the most widely used method for...
12:19
Bacterial Transformation: Electroporation
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2023
The term “transformation” refers cellular ingestion of foreign DNA. In nature, transformation can occur in certain types of bacteria. In molecular biology, however, transformation is artificially induced through the creation of pores in the bacterial cell walls. Bacterial cells that are able to take up DNA from the environment are called competent cells. Electrocompetent cells can be produced in the laboratory and transformation of these cells can be achieve via the application of an...
FAQs
The electrical pulses temporarily alter the hippocampal cell membrane rather than permanently damaging it. Transient pores provide an entry route for DNA, RNA, or other molecular tools, after which the membrane reseals. This sequence is important because it combines short-lived membrane permeability with the possibility of longer-lasting gene expression or experimental effects inside targeted cells.
Different molecular cargos support different experimental goals. DNA can enable gene expression, whereas RNA or other molecular tools can produce other intended cellular effects. Selecting among them allows investigators to manipulate hippocampal cells according to the question being studied, such as examining neuronal development, circuit behavior, or molecular mechanisms associated with memory.
Targeting hippocampal neurons emphasizes the function and plasticity of established neural circuits, while targeting progenitor cells focuses more directly on developmental processes and the formation of new neural cells. This distinction lets researchers connect molecular manipulation with either circuit-level phenomena, such as memory-related activity, or cellular changes occurring during hippocampal development.
A study first identifies the hippocampal tissue or experimental animal model and the cell population to be examined. The selected DNA, RNA, or molecular tool is then applied while brief electrical pulses promote cellular entry. After membrane resealing, investigators examine gene expression or other experimental effects to determine how the manipulation altered hippocampal cells or circuits.
Researchers can use the method when they need targeted molecular manipulation of hippocampal cells in developing tissue or experimental animal models. It supports studies of neuronal development, synaptic or circuit plasticity, learning, memory, and spatial processing. The same approach also contributes to models of neurological disorders and to investigations of potential therapeutic strategies.
The resulting analysis can focus on whether introduced DNA produces gene expression, whether RNA or another molecular tool produces a desired cellular effect, or whether manipulated cells alter hippocampal circuit behavior. These outcomes help connect molecular changes with broader neuroscience questions, including how hippocampal networks contribute to memory, spatial processing, development, and disease-related mechanisms.