28,022 Views
•
06:00 min
•
January 18, 2016
DOI:
Summary
Automatically generated
This protocol describes in detail how to specifically transfect different regions in the C57BL/6 central nervous system via in utero electroporation. Included in this protocol are detailed instructions for transfections of regions that develop into the cortex, hippocampus, thalamus, hypothalamus, lateral septal nucleus and striatum.
Related Videos
26:17
In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos
Related Videos
27784 Views
13:12
Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
Related Videos
19821 Views
09:30
Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
Related Videos
42945 Views
19:45
Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection
Related Videos
16817 Views
25:06
In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells
Related Videos
18817 Views
11:48
Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
Related Videos
15825 Views
06:42
Subtype-selective Electroporation of Cortical Interneurons
Related Videos
8833 Views
10:49
In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity
Related Videos
9621 Views
10:45
Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
Related Videos
7385 Views
08:05
Murine In Utero Electroporation
Related Videos
20384 Views
Read Article
Cite this Article
Baumgart, J., Baumgart, N. Cortex-, Hippocampus-, Thalamus-, Hypothalamus-, Lateral Septal Nucleus- and Striatum-specific In Utero Electroporation in the C57BL/6 Mouse. J. Vis. Exp. (107), e53303, doi:10.3791/53303 (2016).
Copy