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Cortex-, Hippocampus-, Thalamus-, Hypothalamus-, Lateral Septal Nucleus- and Striatum-specific In Utero Electroporation in the C57BL/6 Mouse
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JoVE Journal Neuroscience
Cortex-, Hippocampus-, Thalamus-, Hypothalamus-, Lateral Septal Nucleus- and Striatum-specific In Utero Electroporation in the C57BL/6 Mouse

Cortex-, Hippocampus-, Thalamus-, Hypothalamus-, Lateral Septal Nucleus- and Striatum-specific In Utero Electroporation in the C57BL/6 Mouse

DOI:
10.3791/53303-v

•

06:00 min

•

January 18, 2016

•

,
1TARC (Translational Animal Research Center),University Medical Center, JGU Mainz

Chapters

  • 00:05Title
  • 01:11Transfection of Specific Cerebral Areas
  • 04:16Results: Angle-map for Electroporation of Specific Areas in the Murine Cortex, Hippocampus, Lateral Septal Nucleus, Striatum, Thalamus and Hypothalamus
  • 05:23Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes in detail how to specifically transfect different regions in the C57BL/6 central nervous system via in utero electroporation. Included in this protocol are detailed instructions for transfections of regions that develop into the cortex, hippocampus, thalamus, hypothalamus, lateral septal nucleus and striatum.

Tags

Keywords: In Utero ElectroporationCortexHippocampusThalamusHypothalamusLateral Septal NucleusStriatumCentral Nervous SystemCell ManipulationBrain DevelopmentCell DifferentiationGenetic ModificationEmbryoLateral VentricleElectrode PlacementTransfection

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