Membrane Seal
Membrane Seal - Related Videos
06:10
Accessible Silicone Chip-to-Membrane Sealing Procedure for Flexible, Reliable Bonding
0 Views •
2026
A novel sealing protocol to produce water-tight silicone-based seals for membranes of Polydimethylsiloxane (PDMS) chip devices. Intended for labs setting-up chip models and small-scale chip platform development. We demonstrate the protocol using plastic and native tissue membranes. This procedure only requires PDMS, toluene, mold, membrane, and a vacuum chamber.
04:41
Optimized Sealing Process and Real-Time Monitoring of Glass-to-Metal Seal Structures
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Cited by 7 •
2019
Key procedures to optimize the sealing process and achieve real-time monitoring of the metal-to-glass seal (MTGS) structure are described in detail. The embedded fiber Bragg grating (FBG) sensor is designed to achieve online monitoring of temperature and high-level residual stress in the MTGS with simultaneous environmental pressure monitoring.
07:51
The C-seal: A Biofragmentable Drain Protecting the Stapled Colorectal Anastomosis from Leakage
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Cited by 8 •
2010
The C-seal is a biofragmentable drain protecting the stapled colorectal anastomosis. In this video, details of the manufacturing, deployment and use are shown.
20:21
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
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Cited by 47 •
2012
Herein is described a robotic approach to high-throughput crystallization of membrane proteins in lipidic mesophases for use in structure determination using macromolecular X-ray crystallography. Three robots capable of handling the viscous and sticky protein-laden mesophase integral to the method are introduced.
22:00
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
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Cited by 48 •
2010
Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic mesophases.
FAQs
A high-resistance junction restricts unwanted ionic movement between the electrode and surrounding membrane. This minimizes leak currents that could obscure the membrane current being measured and reduces electrical noise in the recording. As a result, changes produced by membrane channels can be detected with greater precision and interpreted more reliably.
Gentle electrode contact positions the recording tip against the cell membrane without disrupting the target region. Applied suction then draws the membrane tightly against the electrode opening, strengthening the junction and helping isolate the membrane area beneath the tip. These coordinated steps create the low-leak environment needed for sensitive current measurements.
Seal quality directly affects signal fidelity because leakage and electrical noise can distort measured currents. A stable seal makes it easier to resolve action potentials, synaptic transmission, ion-channel behavior, and broader membrane properties. Reliable measurements are therefore important for connecting cellular electrical mechanisms with neural communication and brain function.
The electrode is brought into gentle contact with the cell membrane, after which suction is applied to pull the membrane firmly against the electrode tip. The resulting junction must remain stable enough to limit ionic leak and noise. Once this condition is achieved, the electrode can support precise measurements of electrical activity.
A stable junction supports recordings of action potentials, synaptic transmission, ion-channel behavior, and membrane properties. These measurements provide different views of how cells generate and respond to electrical signals, from channel-level currents to activity involved in communication between neurons. The appropriate outcome depends on which electrical process the experiment targets.
These recordings are useful in neurons and other excitable cells, where electrical activity reflects membrane mechanisms. By isolating currents through membrane channels, investigators can examine how those mechanisms contribute to signaling between cells. In neuroscience, the resulting measurements help relate cellular electrical behavior to neural communication and broader brain function.