Gfp-positive Neurons
Gfp-positive Neurons - Related Videos
09:14
Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
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Cited by 5 •
2012
In this protocol, we update recent progress in imaging Ca2+ signals of GFP-tagged neurons in brain tissue slices using a red fluorescent Ca2+ indicator dye.
09:30
Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices
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Cited by 3 •
2010
Activity in neuronal systems often requires synchronous action potential discharges from neurons within a specific population. For example, pulses of gonadotropin-releasing hormone (GnRH) likely require coordinated activity between GnRH neurons. We present our methodological approach for reliably obtaining simultaneous electrophysiological recordings from the diffusely distributed GnRH neurons.
07:55
Imaging the Intracellular Trafficking of APP with Photoactivatable GFP
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Cited by 6 •
2015
While the transport of cell surface proteins is relatively easily studied, visualizing the trafficking of intracellular proteins is much more difficult. Here, we use constructs incorporating photoactivatable GFP and demonstrate a method to accurately follow the amyloid precursor protein from the Golgi apparatus to down-stream compartments and follow its clearance.
07:30
Electrophysiological Characterization of GFP-Expressing Cell Populations in the Intact Retina
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Cited by 5 •
2011
This article depicts the recording of individual cells from fluorescently tagged neuronal populations in the intact mouse retina. By using two-photon infrared excitation transgenetically labeled cells were targeted for patch-clamp recording to study their light responses, receptive field properties, and morphology.
07:06
Positive Reinforcement Studies
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2023
Researchers study learning of a behavior through the use of operant conditioning. This type of learning involves associating the behavior with a consequence, which is a reward or punishment. If the consequence is a reward, it leads to reinforcement of the desired behavior. One type of reinforcement approach is positive reinforcement, where the behavior is rewarded with an artificial, natural, or social reinforcer. Studies using positive reinforcement as a tool can help tease out important...
FAQs
At the molecular level, GFP fluorescence depends on its chromophore. After the chromophore absorbs light at appropriate wavelengths, it emits light at longer wavelengths, producing the detectable green signal. Fluorescence microscopy captures this emitted signal, allowing investigators to distinguish expressing neurons from surrounding tissue and determine where the labeled cells are located.
Expression serves as an interpretive link between fluorescence and the experimental design. If GFP expression is associated with a cell type, genetic element, or treatment, the labeled population can be examined in that specific context. This relationship helps researchers connect a visible neuronal group with its biological classification or experimental condition rather than treating fluorescence as an arbitrary marker.
GFP labeling can expose neuronal morphology, including the visible form and organization of labeled cells, while also supporting examination of their projections. Those projections help researchers follow how labeled populations extend through neural tissue. This makes the signal useful not only for locating cells, but also for relating cellular structure to the organization of neuronal pathways.
A basic analysis workflow begins by locating fluorescent cells with fluorescence microscopy. Researchers can then examine the labeled neurons’ morphology, follow the distribution of cell populations or projections, and identify cells suitable for further study. The workflow moves from visual localization to structural or population-level analysis, while preserving the connection between the observed signal and the selected experimental labeling strategy.
After fluorescence identifies a neuron of interest, researchers can select that individual cell for physiological or molecular analysis. The label provides a visual criterion for targeting the cell, while the subsequent study addresses properties not established by fluorescence alone. This separation lets investigators combine anatomical identification with other measurements in the same experimental design.
Complex circuits contain many neurons whose relationships can be difficult to distinguish visually. GFP-linked labeling allows researchers to follow selected populations and their projections, connecting individual cells with broader circuit organization. Selecting particular neurons for physiological or molecular studies adds cellular-level information, supporting investigations that relate neuronal identity, structure, and experimental condition to organization and behavior within neural circuits.