Optical Sensor Measurement
Optical Sensor Measurement - Related Videos
09:48
Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping
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Cited by 8 •
2016
We demonstrate use of a fiber optic distributed sensor for mapping the temperature field of mixing air jets. The Rayleigh scattering-based sensor generates thousands of data points along a single fiber to provide exceptional spatial resolution that is unattainable with traditional sensors such as thermocouples.
08:32
Development of Whispering Gallery Mode Polymeric Micro-optical Electric Field Sensors
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Cited by 7 •
2013
A high-sensitivity photonic micro sensor was developed for electric field detection. The sensor exploits the optical modes of a dielectric sphere. Changes in the external electric field perturb the sphere morphology leading to shifts in its optical modes. The electric field strength is measured by monitoring these optical shifts.
09:04
Epidural Intracranial Pressure Measurement in Rats Using a Fiber-optic Pressure Transducer
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Cited by 30 •
2012
A novel technique to record the pressures within the skull is described. The minimally invasive method uses a fibre-optic pressure sensing system to accurately measure intracranial pressure (ICP) in anaesthetized rats without causing significant brain trauma. The technique may be used in a wide range of experimental models.
11:39
Non-invasive Optical Measurement of Cerebral Metabolism and Hemodynamics in Infants
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Cited by 49 •
2013
We combined frequency-domain near-infrared spectroscopy measures of cerebral hemoglobin oxygenation with diffuse correlation spectroscopy measures of cerebral blood flow index to estimate an index of oxygen metabolism. We tested the utility of this measure as a bedside screening tool to evaluate the health and development of the newborn brain.
09:39
Optical Biosensing
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2023
Optical biosensors utilize light to detect the binding of a target molecule. These sensors can utilize a label molecule, which produces a measurable signal such as fluorescence, or these sensors can be label-free and use the changes in optical properties, such as refractive index, to sense for the binding of the target molecule. This video introduces both label and label-free optical biosensors, demonstrates their use in the laboratory, and shows some applications of the technology.
FAQs
An indicator converts a biological event into a measurable optical change. Fluorescent molecules may alter their intensity or wavelength when they bind a target molecule, whereas voltage-responsive indicators react to membrane voltage. The photodetector then records that change, allowing the optical readout to be related to neural or cellular activity after calibration.
Calibration establishes how recorded light relates to the signal being measured. In optical sensor measurement, this step is important because detector output must be interpreted alongside the indicator’s intensity or wavelength response. A calibrated measurement supports quantitative comparisons of neural activity, intracellular calcium, neurotransmitter dynamics, or tissue oxygenation across experimental conditions.
These light interactions provide different routes for detecting a physical or biological change. A sensor may analyze emitted light from a fluorescent indicator, changes in absorption, reflected light, or scattered light. The selected optical response determines how the detector captures information and helps connect changes in light to the underlying biological or physical signal.
A neuroscience workflow begins by selecting an optical indicator that responds to the event of interest, such as a target molecule or membrane voltage. The resulting light change is collected by a photodetector, and the system is calibrated so the recorded intensity or wavelength can be interpreted quantitatively. The resulting signal is then related to cellular or neural activity.
Optical sensor measurement can track several types of neural and tissue signals, including neural activity, intracellular calcium, neurotransmitter dynamics, and tissue oxygenation. These readouts connect optical changes with events occurring at cellular or circuit levels. Consequently, the approach can support experiments examining brain function, disease mechanisms, and responses to experimental treatments.
High spatial and temporal resolution helps relate an optical signal to both where an event occurs and when it changes. In neuroscience, that combination supports connections between cellular activity and broader circuit-level events. It therefore strengthens investigations of brain function and provides a basis for examining disease-related changes or responses to experimental treatments.