Periventricular Region
Periventricular Region - Related Videos
04:30
Isolation of Neural Stem Progenitor Cells from the Spinal Cord Periventricular Region of an Adult Rat
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2025
This video presents a method for isolating neural stem/progenitor cells from the periventricular tissue of the spinal cord in adult rats. The technique includes incubating the tissue fragments in a solution containing proteolytic enzymes to disrupt the extracellular matrix and release the cells. Subsequently, the released cells are plated in a medium containing specific growth factors that enhance neural stem/progenitor proliferation and promote aggregation into three-dimensional neurospheres.
08:26
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
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Cited by 23 •
2015
The adult mammalian spinal cord contains neural stem/progenitor cells (NSPCs) that can be isolated and expanded in culture. This protocol describes the harvesting, isolation, culture, and passaging of NSPCs generated from the periventricular region of the adult spinal cord from the rat and from human organ transplant donors.
01:44
Establishing a Co-Culture of Interneurons with Periventricular Endothelial Cells
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2025
This video demonstrates a technique for establishing a co-culture of interneurons with perivascular endothelial cells. The cells are added to a one-well insert placed inside a culture dish. After the cells adhere to the dish, the insert is removed, allowing the cells to proliferate and interact and form a co-culture.
02:19
Assessing Interneuron Response to Chemoattractive Cues from Periventricular Endothelial Cells
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2025
This video shows a method to test interneuron migration in response to chemoattractive cues from periventricular endothelial cells (PVECs). Interneurons are placed in a central compartment, with PVECs and control cells on either side. Increased migration towards PVECs indicates a positive response to their chemoattractive signals.
06:24
Mouse Models of Periventricular Leukomalacia
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Cited by 28 •
2010
We established mouse models of periventricular leukomalacia (PVL), the predominant brain injury in premature infants characterized by periventricular white matter lesions. Hypoxia/ischemia with/without systemic infection are the primary causes of PVL. Unilateral carotid ligation and hypoxia exposure with/without lipopolysaccharide injection creates PVL-like lesions in P6 mice.
FAQs
Cerebrospinal fluid can serve as a local source of chemical signals, nutrients, and waste-related influences for tissue bordering the ventricles. Cells near the ventricular lining respond within this closely situated environment, linking fluid composition with the state of adjacent neural tissue. This relationship gives neuroscientists a way to study how fluid regulation affects local nervous-system function.
The ventricular lining forms an important interface between cerebrospinal fluid and surrounding nervous tissue. Its neighboring cells help determine how substances in the fluid can influence nearby regions, making this boundary biologically active rather than merely structural. Examining that interface helps researchers connect changes in the ventricular environment with effects on adjacent neural tissue.
Neural stem and progenitor cell activity is supported in selected periventricular regions, not necessarily uniformly throughout the entire area. This regional pattern matters because stem cells and progenitors can be studied in relation to local cerebrospinal-fluid and ventricular-boundary conditions. Their presence makes the region relevant to questions about how neural cells are generated during nervous-system development.
Because the periventricular region contains nervous tissue directly exposed to influences from the ventricular boundary and cerebrospinal fluid, alterations there may affect both fluid regulation and adjacent neural structures. This proximity is especially important when interpreting disease-related changes, since abnormalities can involve the ventricular boundaries, nearby white matter, or both rather than a single isolated compartment.
Studies of the periventricular region can relate ventricular-environment signals and selected stem or progenitor-cell activity to broader patterns of brain development. The region provides a context for asking how local conditions influence developing nervous tissue and neurogenesis. Findings can connect cellular activity at ventricular boundaries with larger developmental processes in the central nervous system.
In hydrocephalus, attention to the periventricular region helps investigators consider how altered ventricular conditions relate to surrounding nervous tissue. This perspective separates questions about cerebrospinal-fluid regulation from changes affecting the adjacent boundary or white matter. Studying both components supports a more precise interpretation of disease-related effects than viewing the ventricles independently.
Periventricular location provides a shared anatomical context for studying disorders that affect ventricular boundaries or nearby white matter. In demyelinating disorders, adjacent white matter is particularly relevant, whereas tumors may involve tissues near the ventricular boundary. Comparing these conditions through their location helps researchers describe disease-related changes while retaining the region’s fluid and neural context.