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The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
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The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

Article DOI: 10.3791/1026-v • 08:16 min • January 6th, 2009
January 6th, 2009 •
1, 1, 1
1Gene Expression Division, Bio-Rad Laboratories, Inc

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This procedure describes how to establish primary hematopoietic cell cultures from murine bone marrow and is followed by transfection using the Gene Pulser MXCell electroporation system.

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Primary Hematopoietic Cell Cultures Murine Bone Marrow Electroporation Plasmid DNA SiRNA Gene Pulser MXcell Electroporation System Gene Pulser Electroporation Buffer Transfection Nucleic Acids Mammalian Cells Difficult-to-transfect Cells Stem Cells Video Demonstration Femur Tibia
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