LAD-Ligation: A Murine Model of Myocardial Infarction

Mandy V.V. Kolk; Danja Meyberg; Tobias Deuse; Karis R. Tang-Quan; Robert C. Robbins; Hermann Reichenspurner; Sonja Schrepfer

doi:10.3791/1438

JoVE Journal
Biology

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JoVE Journal Biology

LAD-Ligation: A Murine Model of Myocardial Infarction

法援署结扎:有心肌梗死的小鼠模型

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08:23 min

October 14, 2009

DOI: 10.3791/1438-v

Mandy V.V. Kolk^1,2, Danja Meyberg^1,2, Tobias Deuse^1,2, Karis R. Tang-Quan^1,2,3, Robert C. Robbins³, Hermann Reichenspurner^1,2, Sonja Schrepfer^1,2,3

¹Transplant and Stem Cell Immunobiology Lab (TSI),University Heart Center Hamburg, ²CVRC,University Hospital Hamburg, ³Department of CT Surgery,Stanford University School of Medicine

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本视频演示了如何使用快捷及可靠的模型来研究心肌缺血的病理生物学和病理生理过程。

进行中线宫颈切口以露出气管，并在气管上切一个小孔以插入气管插管。将鼠标转向右侧，在第三和第四肋骨之间进行左侧开胸手术。心脏暴露出来，LAD 用单针结扎。

放置胸管并关闭切口后，通过小心抽吸注射器引流胸部。最后，鼠标打开。它的背面。

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