Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Daniel L. Floyd; Stephen C. Harrison; Antoine M. van Oijen

doi:10.3791/1484

JoVE Journal
Biology

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JoVE Journal Biology

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

病毒融合动力学测量方法在单粒子能级

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14:59 min

September 7, 2009

DOI: 10.3791/1484-v

Daniel L. Floyd¹, Stephen C. Harrison^1,2, Antoine M. van Oijen¹

¹Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School, ²Howard Hughes Medical Institute,Harvard Medical School

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我们提出了一个

该测定中使用的盖玻片用糊精功能化，然后掺入微流体流动池中。接下来，每个流动池与脂质体悬浮液一起孵育，脂质体悬浮液融合，在水合的糊精顶部形成脂质双层。在荧光显微镜下，加入荧光 4 标记的病毒和荧光素标记的链霉亲和素，并允许病毒对接到双层。

酸性缓冲液的流入会启动病毒与双层的融合，数码相机会记录整个检测过程中的荧光变化。大家好，我是 Daniel Floyd，在哈佛大学生物化学和分子药理学系 Anine Vanian 的实验室工作。今天，我将向您展示一种在单粒子水平上可视化病毒融合动力学的方法。

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