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Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

Article DOI: 10.3791/1995-v • 11:22 min • June 26th, 2010
June 26th, 2010 •
1, 1
1Department of Molecular Physiology and Biophysics, Vanderbilt University

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This protocol describes a general approach to perform photoconversion of fluorescent proteins on a confocal laser scanning microscope. We describe procedures for the photoconversion of puried protein samples, as well as for dual-probe optical highlighting in live cells with mOrange2 and Dronpa.

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Photoconversion Fluorescent Proteins Optical Highlighter Live Cells Marking Subpopulation Tracking Intensity Exposure Time Photoconversion Light Photobleach Confocal Laser Scanning Microscope Purified Protein Droplet Samples Photophysical Behavior Microscope Configuration Dual-probe Optical Highlighting MOrange2 Dronpa
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