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Chapters
- 00:01Introduction
- 00:24Preparation of Mosquitoes and Work Area
- 01:47injecting Mosquitoes with dsRNA
- 08:53Treatment of Mosquitoes after Injection
- 11:11Conclusion
- 11:37End Credits
Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the Dimopoulos lab to inject dsRNA into Anopheles gambiae mosquitoes, which harbor the malaria parasite. The technique manipulating the injection setup and injecting dsRNA into the thorax is illustrated.
