高通量 microRNA 表达谱分析：在 Fluidigm Dynamic ArrayTM IFCs 上进行纳升级优化多重 qRT-PCR
23.4K 次观看
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被引用 13 次
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07:27 分钟
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2011年8月3日
DOI :10.3791/2552-v
2011年8月3日
•23.4K 次观看
Felix Moltzahn1,2,3 , Nathan Hunkapiller1,2,4 , Alain A. Mir5 , Tal Imbar1,2,6 , Robert Blelloch1,2,3,7
1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco , 2Center for Reproductive Sciences, University of California San Francisco , 3Department of Urology, University of California San Francisco , 4Department of Cell and Tissue Biology, University of California San Francisco , 5Fluidigm Corporation, Fluidigm Corporation , 6Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, 7UCSF - Helen Diller Family Comprehensive Cancer Center, University of California San Francisco
本文介绍了一种优化的多重逆转录定量PCR（qRT-PCR）方案，结合微流控平台，作为检测microRNA（miRNA）表达水平的高通量筛选工具，具有成本低、耗时短的优点，尤其适用于样本量有限的情况。
Chapters in this video
0:05
Title
0:55
RNA Concentration
2:03
Gel Purification of pre-PCR Product
3:46
Preparing the 96.96 Dynamic Array IFC, the Samples, and the 13x Assays
5:25
qRT-PCR Analysis of miRNA Expression in Mouse ESC and Human Serum
6:10
qRT-PCR Analysis
7:04
Conclusion
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