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Etichettatura stirpe di cellule Zebrafish con laser Uncagable Fluoresceina Destrano

Article DOI: 10.3791/2672-v • 07:36 min • April 28th, 2011
April 28th, 2011 •
1, 2, 2, 1
1Department of Biological Sciences, Vanderbilt University, 2Department of Chemical and Systems Biology, Stanford University

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Questo protocollo delinea un modo per etichettare e tracciare il destino dei piccoli gruppi di cellule di embrioni di zebrafish con UV-uncaging della fluoresceina in gabbia, seguita da montare tutto immunomarcatura per amplificare il segnale proveniente dal fluoresceina Uncaged.

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Biologia dello Sviluppo Numero 50 zebrafish etichettatura lignaggio in gabbia fluoresceina transgene
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