High-throughput Screening and Biosensing with Fluorescent <em>C. elegans</em> Strains

Chi K. Leung; Andrew Deonarine; Kevin Strange; Keith P. Choe

doi:10.3791/2745

JoVE Journal
Biology

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High-throughput Screening and Biosensing with Fluorescent C. elegans Strains

高通量筛选和生物传感与荧光 C。线虫菌株

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14:53 min

May 19, 2011

DOI: 10.3791/2745-v

Chi K. Leung¹, Andrew Deonarine¹, Kevin Strange², Keith P. Choe¹

¹Department of Biology,University of Florida, ²Mount Desert Island Biological Laboratory

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液为基础的培养和分配过程

本视频演示了一种培养和分配 CL gans 荧光菌株的方法，用于化学库的高通量筛选或环境污染物检测，以评估化学或环境试剂对特定蛋白质活性的影响。在 CL elgan 中，首先制备细菌培养物作为蠕虫和 GFP 转基因的食物。培养同步蠕虫。

然后收集 0.5 至 200 万条蠕虫，洗涤并分配到 3 84 中。将微量滴定板孔板，然后将化学库中的小分子或测试样品（如水、食物或土壤）添加到蠕虫中。最后，用酶标仪测量蠕虫的荧光。

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