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Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography

Article DOI: 10.3791/3119-v • 07:52 min • September 22nd, 2011
September 22nd, 2011 •
1, 1, 1,2,3, 1,2,3
1Julius L. Chambers Biomedical/Biotechnology Research Institute, North Carolina Central University, Durham, 2Department of Biology, North Carolina Central University, Durham, 3Department of Physiology & Pharmacology and Hypertension & Vascular Research Center, Wake Forest University School of Medicine

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An automated myography method for force measurements in isolated mesenteric arteries is described. It employs a Mulvany-Halpern Auto Dual Wire Myograph 510A to determine responses to phenylephrine and extracellular calcium. The method allows consistent determination of isometric responses to agonists in small vessels of diameters of 60 - 300 μm, independently.

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Mesenteric Artery Contraction Relaxation Automated Wire Myography Proximal Resistance Vessels Blood Flow Rat Mesenteric Artery Myography Technique Isometric Conditions Force Production Sensitivity Agonists Stretch Compliance Of Mounting Wires Stainless Steel Wires Tungsten Wires Oxidation Recorded Responses Vascular Smooth Muscle Cell Receptors Phenylephrine Extracellular Calcium (Ca2+e)
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