RNAi Screening to Identify Postembryonic Phenotypes in <em>C. elegans</em>

Katherine K. Beifuss; Tina L. Gumienny

doi:10.3791/3442

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Biology

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RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

RNAi筛选识别胚后的表型 C。线虫

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09:40 min

February 13, 2012

DOI: 10.3791/3442-v

Katherine K. Beifuss¹, Tina L. Gumienny¹

¹Department of Molecular and Cellular Medicine,Texas A&M University System Health Science Center

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我们描述了致敏的方法来确定胚后调节蛋白表达和定位

该程序的目标是使用基于 RNAi 的基因组筛选和荧光标记的蛋白质来识别 C 优雅中蛋白质表达和定位的胚胎后调节因子。一旦构建了合适的筛选菌株，就选择了 RNAi 文库。该程序的第一步是将文库克隆包装到细菌中。

然后选择细菌，培养并喂给 stage c elegance 线虫。让线虫生长 72 小时后，观察它们感兴趣的表型的变化。最终，用荧光显微镜观察受攻击蛋白质的表达或亚细胞定位的变化。

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