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Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System
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September 16, 2011
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Summary
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We have developed a technique to test protein-protein interactions in plant. A yellow fluorescent protein (YFP) is split into two non-overlapping fragments. Each fragment is cloned in-frame to a gene of interest via Gateway system, enabling expression of fusion proteins. Reconstitution of YFP signal only occurs when the inquest proteins interact.
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Protein InteractionsPlantGateway CompatibleBimolecular Fluorescence ComplementationBiFC SystemYellow Fluorescent ProteinIn Vivo Interaction TestingNicotiana BenthamianaAgrobacterium Mediated TransformationFusion ProteinsYFP Signal ReconstitutionYeast Two Hybrid AssayIndirect InteractionsGateway TechnologyGene Of Interest (GOI)Expression And Functional Analysis SystemsOrientation And Reading Frame MaintenanceExpression-ready ClonesBiFC And Y2H VectorsEase Of Use
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Tian, G., Lu, Q., Zhang, L., Kohalmi, S. E., Cui, Y. Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System. J. Vis. Exp. (55), e3473, doi:10.3791/3473 (2011).
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