Journal
/
医学
/
Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain
JoVE 杂志
医学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 医学
Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain

Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain

10,661 Views

•

11:29 min

•

October 03, 2012

DOI:

10.3791/3788-v

DOI:
10.3791/3788-v

•

11:29 min
October 03, 2012

•

4 Views
, ,
1Department of Pathology, National Prion Disease Pathology Surveillance Center,Case Western Reserve University School of Medicine, 2Department of Neurology, National Prion Disease Pathology Surveillance Center,Case Western Reserve University School of Medicine

Summary

Automatically generated

A new species of cellular prion protein (PrPC) has recently been identified in uninfected human brains using the methods described here. These methods can be used to isolate various PrP species, while some of them are also useful in isolating other misfolded protein aggregates from human brains.

Explore More Videos

Soluble PrP OligomersInsoluble PrP OligomersNormal Human BrainPrPCPrPScDetergent-solubleProteinase K (PK)-digestionDetergent-insoluble AggregatesConformational Transitionα-helical Structuresβ-sheet StructuresEndogenous PrPScIntermediate PrPSilent PrionBiophysical ApproachesBiochemical ApproachesUltracentrifugationSucrose Step Gradient SedimentationSize Exclusion ChromatographyIPrP EnrichmentGene 5 Protein (g5p)PK-treatment

Read Article

Cite this Article

Xiao, X., Yuan, J., Zou, W. Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain. J. Vis. Exp. (68), e3788, doi:10.3791/3788 (2012).

Download .ris file

Copy

Share Video

.

Copy