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Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

Article DOI: 10.3791/4221-v • 11:31 min • October 31st, 2012
October 31st, 2012 •
*1,2, *1,3, 1, 1, 1, 1,4, 5, 1, 1
1Department of Surgery, Stanford University, 2Department of Surgery, Duke University, 3Department of Surgery, Saint Joseph Mercy Hospital, 4School of Medicine, University of California, San Francisco, 5School of Dentistry, University of California, Los Angeles
* These authors contributed equally

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总结概括
Automatic Translation

This protocol describes the isolation of adipose-derived stromal cells from lipoaspirate and the creation of a 4 mm critical-sized calvarial defect to evaluate skeletal regeneration.

Tags

Repair Critical-sized Calvarial Defect Adipose-derived Stromal Cells Lipoaspirate Craniofacial Skeletal Repair Tissue Formation Cell-based Approach Stem Cells Osteogenic Potential Scaffolding Biomaterials Cellular Delivery Osteoconductive Hydroxyapatite-coated Poly(lactic-co-glycolic Acid) HA-PLGA Scaffold Osseous Regeneration Translational Approaches Bone Tissue Regeneration Progenitor Cell Tissue Defect
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