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Immunology and Infection

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Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

Article DOI: 10.3791/4322-v • 14:48 min • December 26th, 2012
December 26th, 2012 •
1,2, 1,2, 3, 2, 1,2, 1
1Research Group Immune Regulation, Helmholtz Centre for Infection Research, 2Research Group Infection Immunology, Institute of Medical Microbiology, Otto-von-Guericke University, 3Department of Experimental Immunology, Helmholtz Centre for Infection Research

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We describe the rapid isolation of primary murine type II alveolar epithelial cells (AECII) by flow cytometric negative selection. These AECII show high viability and purity and are suitable for a wide range of functional and molecular studies regarding their role in respiratory conditions such as autoimmune or infectious diseases.

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Flow Cytometric Isolation Primary Murine Type II Alveolar Epithelial Cells Functional Studies Molecular Studies Immune Regulation Cytokine Production Antigen-presenting Cells Infection T-cell Mediated Autoimmunity Clinical Contexts Airway Hyper-reactivity Infections Cell Lines Large Numbers Of Cells Complex Processes Autoimmune Inflammation
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