Name: 2012年5月：这个月在朱庇特 (视频) | JoVE Journal
Uploaded: 2012-05-01T13:00:00.000+00:00
Duration: 5 min 29 s
Description: 了解JoVE期刊2012年5月号中神经科学与生物学领域的创新研究方法。本期通过视频演示展示前沿实验技术，为研究人员提供可重复的科学资源共享平台。

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5 minutes

<h2 class="jove_title"><a href="https://www.jove.com/video/3436">MPI CyberMotion Simulator：实现一种新型运动模拟器，以研究三维多传感器路径集成</a></h2>
Michael Barnett-Cowan1， Tobias Meilinger1， Manuel Vidal2， Harald Teufel1， Heinrich H. Bülthoff1， 3 1马克斯·普朗克生物控制论研究所人类感知、认知和行动系，阿拉伯数字法国学院感知与行动生理学实验室 - 高丽大学脑与认知工程系 3CNRS
描述了一种深入了解人类如何在三维空间中导航的有效方法。该方法利用了运动模拟器，能够以传统模拟器无法实现的方式移动观察者。结果证实，水平面上的运动被低估了，而垂直运动被高估了。
<h2 class="jove_title"><a href="https://www.jove.com/video/3586">他莫昔芬通过7-脱氧泛克拉他汀的新型合成 C-1 类似物增强人乳腺癌和神经母细胞瘤细胞中的凋亡和自噬诱导</a></h2>
Dennis 马1， Jonathan Collins2， Tomas Hudlicky2， Siyaram Pandey1 1温莎大学化学与生物化学系， 2布鲁克大学化学系和生物技术中心
我们合成了一种新的普雷司他汀类似物，具有与天然普雷他汀相当的抗癌活性;有趣的是，他莫昔芬的联合治疗通过线粒体靶向产生了凋亡和自噬诱导的显著增强，而对非癌性成纤维细胞的影响最小。因此，JCTH-4 与他莫昔芬联合使用可以提供一种安全的抗癌疗法。
<h2 class="jove_title"><a href="https://www.jove.com/video/3730">人真菌类味觉细胞的分离和培养</a></h2>
Hakan Ozdener1， Andrew I. Spielman2， Nancy E. Rawson3 1Monell Chemical Senses Center， 2纽约大学牙科学院， 3AFB International
我们的目标是开发一种可重复的方案，用于分离和维持人类真菌状味道细胞的长期培养。通过活检获得的人真菌状细胞在培养物中成功维持超过 8 次传代（12 个月），而没有活力损失。
<h2 class="jove_title"><a href="https://www.jove.com/video/3837">评估果蝇幼虫热伤害感受的局部和全局方法 </a></h2>
Abanti Chattopadhyay1，*， A'Tondra V. Gilstrap1， 2，*， Michael J. Galko1， 3， 4， 5 1德克萨斯大学 MD 安德森癌症中心生物化学与分子生物学系，2休斯顿大学市中心学者学院/MARC 学者，3基因与发育研究生课程，德克萨斯大学 MD 安德森癌症中心，4神经科学研究生课程，德克萨斯大学生物医学科学研究生院，5基因与发育研究生课程，德克萨斯大学生物医学科学 研究生院*这些作者的贡献相同
在本文中，我们演示了研究果蝇幼虫热伤害感受的分析方法。一种检测涉及对热伤害感受器1,2 的空间限制（局部）刺激，而第二种涉及大部分或全部此类神经元的批发（全局）激活3。总之，这些技术允许可视化和量化果蝇伤害感受感觉神经元的行为功能。
<h2 class="jove_title"><a href="https://www.jove.com/video/3903">自己动手回收意外掉落到低温储罐中的冻存样品</a></h2>的设备
Rohini Mehta1， 2， 3， <font color='black'>Ancha Baranova1， 2， 3， 4， Aybike Birerdinc1， 2， 3 1乔治梅森大学分子与微生物学系，2乔治梅森大学肝病基因组学研究中心，3Inova Health System转化研究所，4医学遗传学研究中心 RAMS
在这里，我们介绍了一种低成本、耐用的低温耐受装置，用于从装满液氮的杜瓦瓶中取出样品。该设备易于构建和模块化设计，使从低温罐中取样的过程安全而简单。
<h2 class="jove_title"><a href="https://www.jove.com/video/3931">电图生物测定作为寄主植物挥发物的筛选工具</a></h2>
John J. Beck， Douglas M. Light， Wai S. Gee 植物霉菌毒素研究，美国农业部，农业研究服务部
通过电图分析测量成脐橙虫 （Amyelois transitella） 触角对单个成分和混合物的电生理反应，快速筛选寄主植物挥发物的方法。
<h2 class="jove_title"><a href="https://www.jove.com/video/4039">人胰腺采集方案</a></h2>
Martha L. Campbell-Thompson， Emily L. Montgomery， Robin M. Foss， Kerwin M. Kolheffer， Gerald Phipps， Lynda Schneider， Mark A. Atkinson 佛罗里达大学病理学、免疫学和实验室医学系
该视频演示了将人胰腺加工成多种存储格式的解剖程序。在整个胰腺区域保持解剖方向，以便定义区域胰岛的组成和密度。

No conflicts of interest declared.

may 2012: this month in jove

May 2012: This Month in JoVE

The May issue of JoVE (Journal of Visualized Experiments) includes a handful of articles that deal with the senses and perception. Specifically, articles involving gustation (taste), olfaction (smell), nociception (pain), and the vestibular senses (balance and inertial movement) will be released.
The vestibular system is important for perceiving inertial movement, and inertial information is an important component of path integration. Path integration is a process by which the brain integrates multisensory information during movement, so that we maintain a sense of our body&#x2019;s position, relative to a starting point. In nature, many organisms make use of this process, in order to find their way back to their nests or burrows after looking for food.
This May, JoVE will publish a method filmed at the Max Plank Institute of Biological Cybernetics , which explores how path integration occurs in three dimensions. Most studies of this phenomenon are conducted in two dimensions, but thanks to a motion simulator with a large range of motion, path integration can be assessed in the horizontal and vertical planes. By controlling the sensory stimuli delivered to subjects in this device, our authors can dissect the relative contributions of visual and inertial information to estimations of starting position.
Through an elegantly designed series of experiments, these investigators find that subjects are more likely to overestimate angles of movement in the horizontal plane versus the verticals. They also find that subjects were slower to point to the starting position when they had to rely only on inertial stimuli. The underlying neuronal mechanism behind path integration is poorly understood, and further experiments using this system will provide insight into how humans maintain spatial orientation during movement, which has implications that range from understanding vestibular disorders and training better pilots.
Concerning gustation, JoVE will publish a procedure for isolating and culturing human fungiform taste cells this May. Back in 2010, we published an article that dealt with isolation of human fungiform papillae &#x2013; a subtype of the tiny stuctures on our tongues that contain tastebuds. While papillae collection is suitable for acute experiments, long-term studies of taste cells require them to be isolated and cultured.
For the first time ever reported, our authors demonstrate the isolation and culture of human fungiform taste cells and show that they can be maintained in culture for up to 8 passages, or one year. The ability to culture these cells for an extended period of time enables studies of proliferation and regeneration, and obviates the need to repeatedly collect papillae from donors.
Regarding olfaction, JoVE will release an article from the Department of Agriculture, which describes an experiment that transforms the antennae of insects into a screening tool for new pesticide alternatives. After isolating plant volatile compounds via gas chromatography and mass spectrometry, these investigators screen the compounds for biological activity using the electroantennagram (EAG).
Because not all of the volatile compounds emitted by plants elicit biological responses, such a bioassay is incredibly important for finding candidate molecules that can affect insect behavior. In turn, behavioral experiments, can determine whether or not a plant volatile can attract an insect. Plant volatile attractants can be used to confuse insects and potentially could serve as a safe alternative to pesticides.
Thermal nociception, or the perception of noxious thermal stimuli, is the focus of an article from the University of Texas this May. Specifically, thermal nociception is investigated in Drosophila larvae using two methods: the heat probe assay and the heat plate assay. Interestingly, for both methods, larvae must first be treated with UV radiation to induce tissue damage and sensitize thermal nociceptive neurons, which usually respond with a very high threshold.
As its name implies, the heat probe assay, uses a custom-built thermal probe to locally administer thermal stimuli. Nociception is quantified by recording the latency between stimulus onset and withdraw behavior. Intuitively, the second method, or heat plate assay, involves placing drosophila larvae on a heating plate within a drop of water to test whole body responses to noxious heat. Upon placement, drosophila exhibit a series of characteristic behaviors including rolling, whipping, or even seizure-like activity. When combined with genetic manipulation, these assays can provide information about the genes underlying thermal nociception &#x2013; a highly conserved process, from larvae to mammals.
Naturally, an exploration of the senses is not all JoVE has to offer this may. Other notable publications involve painting the pancreas to maintain orientation during histology, using an automated operant device to test multi-cognitive functions in mice, examining tamoxifen-induced apoptosis using timelapse video microscopy, and creating a convenient tool for fishing out lost samples from liquid nitrogen tanks. Stay tuned.

Here are some highlights from the May 2012 Issue of Journal of Visualized Experiments (JoVE).

The May issue of JoVE (Journal of Visualized Experiments) includes a handful of articles that deal with the senses and perception.   Specifically, ...

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2012年5月：​​这个月在朱庇特

了解JoVE期刊2012年5月号中神经科学与生物学领域的创新研究方法。本期通过视频演示展示前沿实验技术，为研究人员提供可重复的科学资源共享平台。

2012年5月：​​这个月在朱庇特 (视频) | JoVE Journal

Aaron Kolski-Andreaco

May 2012: This Month in JoVE

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