An Isolated Semi-intact Preparation of the Mouse Vestibular Sensory Epithelium for Electrophysiology and High-resolution Two-photon Microscopy

Victoria W. K. Tung; Stefano Di Marco; Rebecca Lim; Alan M. Brichta; Aaron J. Camp

doi:10.3791/50471

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

An Isolated Semi-intact Preparation of the Mouse Vestibular Sensory Epithelium for Electrophysiology and High-resolution Two-photon Microscopy

一个孤立的半完整的上下游鼠标前庭上皮细胞电生理和高分辨率的双光子显微镜

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11:52 min

June 13, 2013

DOI: 10.3791/50471-v

Victoria W. K. Tung¹, Stefano Di Marco¹, Rebecca Lim², Alan M. Brichta², Aaron J. Camp¹

¹Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School,University of Sydney, ²School of Biomedical Sciences and Pharmacy,University of Newcastle

Please note that some of the translations on this page are AI generated. Click here for the English version.

前庭毛细胞功能分析是复杂的，最难的部分颅骨，颞骨岩部内位置较深。功能最全的毛细胞的研究已经用毛细胞急性分离。在这里，我们描述了一个半完整的小鼠前庭上皮细胞的电生理和双光子显微镜研究编制。

该程序的总体目标是获得小鼠前庭感觉上皮的近乎正常的准备，可用于研究前庭毛细胞的基本细胞和亚细胞过程。这是通过首先将骨前庭迷路与颅穹窿隔离来实现的。第二步是用细镊子小心地刮掉骨迷路，露出下面的膜迷路，然后将其从骨壳中完全取出。

接下来，通过使用细虹膜剪刀切掉覆盖的膜来暴露前庭感觉上皮。最后一步是将半完整的感觉上皮制剂安装在玻璃底室上，并用加重尼龙网稳定它。最终，膜片钳电生理学和双光子显微镜分别用于表征每种类型的前庭毛、细胞和/或原代腺的内在膜特性和钙信号谱。

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