Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins

Andrius Masedunskas; Natalie Porat-Shliom; Muhibullah Tora; Oleg Milberg; Roberto Weigert

doi:10.3791/50558

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Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins

活体显微镜成像的亚细胞结构的活体小鼠表达荧光蛋白

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08:23 min

September 1, 2013

DOI: 10.3791/50558-v

Andrius Masedunskas^1,2, Natalie Porat-Shliom¹, Muhibullah Tora¹, Oleg Milberg^1,3, Roberto Weigert¹

¹Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch National Institute of Dental and Craniofacial Research,National Institutes of Health, ²Department of Biology,University of North Carolina at Chapel Hill , ³Department of Chemical & Biochemical Engineering and Department of Biomedical Engineering,Rutgers University

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活体显微镜是一个功能强大的工具，使活体动物成像的各种生物过程。在本文中，我们提出了用于成像的亚细胞结构，如分泌颗粒，在活体小鼠的唾液腺的动力学的详细方法。

该程序的总体目标是可视化小鼠唾液腺中的亚细胞结构。这是通过首先手术暴露唾液腺而不损坏它们来实现的。在第二步中，将麻醉的动物固定在显微镜载物台上。

接下来，仔细定位唾液腺，以免在最后一步影响其功能。鼠标使用定制支架进行稳定，以最大限度地减少由于动物的心跳或呼吸而导致的任何运动伪影。最终，亚细胞结构的动力学可以通过活体显微镜成像。

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