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Neuroscience
在分析癫痫样活动和运动的一种低成本方法果蝇
在分析癫痫样活动和运动的一种低成本方法果蝇
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
A Low-cost Method for Analyzing Seizure-like Activity and Movement in Drosophila

在分析癫痫样活动和运动的一种低成本方法果蝇

Full Text
14,122 Views
09:06 min
February 19, 2014

DOI: 10.3791/51460-v

Bryan Stone1, Brian Burke1, Joseph Pathakamuri1, John Coleman2, Daniel Kuebler1

1Department of Biology,Franciscan University of Steubenville, 2Department of Computer Science,Franciscan University of Steubenville

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Please note that some of the translations on this page are AI generated. Click here for the English version.

使用自由和廉价的软件程序的网络摄像头和相结合的,在果蝇机车图案可以被分析以检测速度,距离,以及各马达的活动时间的差别。

该程序的总体目标是识别果蝇运动或癫痫发作行为的改变。这是通过首先使用麻醉将果蝇分离到单独的小瓶中来实现的。接下来,使用一个简单的网络摄像头来记录 Flies 的运动。

然后将数据导入 Image J 以开始分析。最后,使用定制的苍蝇分析软件来确定记录期间移动的距离、速度和持续时间。最终,该测定可以显示与遗传和药理学作相关的运动或癫痫发作行为的变化。

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