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A Modified In vitro Invasion Assay to Determine the Potential Role of Hormones, Cytokines and/or Growth Factors in Mediating Cancer Cell Invasion

Article DOI: 10.3791/51480-v • 06:36 min • April 24th, 2015
April 24th, 2015 •
*1, *2, *2, 2, 2, 2, 2, 2
1Department of Cell Stress Biology, Roswell Park Cancer Institute, 2Department of Pharmaceutical, Social and Administrative Sciences, School of Pharmacy, D'Youville College
* These authors contributed equally

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This video article describes a modified in vitro method to examine the role of hormones, cytokines and/or growth factors in driving cancer cell invasion.

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Modified In Vitro Invasion Assay Potential Role Hormones Cytokines Growth Factors Cancer Cell Invasion Chemoattractant Migration Intravasation Microvessels Targets Cell Migration Invasion Index Specific Hormone Growth Factor Cytokine Invasive Potential Fetal Bovine Serum (FBS) Charcoal-stripped FBS (CS-FBS) Molecule X Change In Cell Invasion Involvement Of Hormones/cytokines/growth Factors Reverse Or Rescue Invasion Phenotype Additive Or Synergistic Effects Driving Or Inhibiting Invasion
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