Primary Culture of Mouse Dopaminergic Neurons

Florence Gaven; Philippe Marin; Sylvie Claeysen

doi:10.3791/51751

Journal

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神经科学

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小鼠多巴胺能神经元的原代培养

Primary Culture of Mouse Dopaminergic Neurons

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神经科学

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JoVE 杂志神经科学

Primary Culture of Mouse Dopaminergic Neurons

小鼠多巴胺能神经元的原代培养

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37,658 Views

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11:58 min

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September 08, 2014

DOI:

10.3791/51751-v

DOI:

10.3791/51751-v

•

11:58 min

September 08, 2014

•

27 Views

Florence Gaven^1,2,3, Philippe Marin^1,2,3, Sylvie Claeysen^1,2,3

¹CNRS, UMR-5203,Institut de Génomique Fonctionnelle, Montpellier, ²Inserm,U661, Montpellier, ³Universités de Montpellier

Summary

Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various stresses.