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珠聚集测定假定的细胞粘附分子的表征
Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

珠聚集测定假定的细胞粘附分子的表征

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08:15 min

October 17, 2014

DOI:

08:15 min
October 17, 2014

10516 Views
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Summary

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Here we present a simple, rapid method for characterizing the intrinsic adhesive properties of putative cell adhesion molecules. The secreted, epitope-tagged ectodomain of a cell adhesion molecule is captured from the culture medium on small, uniform functionalized beads. These beads can then be used immediately in simple bead aggregation assays.

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