Protein Isolation from the Developing Embryonic Mouse Heart Valve Region

Laura A. Dyer; Yaxu Wu; Cam Patterson

doi:10.3791/51911

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Biology

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Protein Isolation from the Developing Embryonic Mouse Heart Valve Region

从发展胚胎小鼠心脏瓣膜区蛋白分离

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06:55 min

September 23, 2014

DOI: 10.3791/51911-v

Laura A. Dyer¹, Yaxu Wu¹, Cam Patterson²

¹McAllister Heart Institute,University of North Carolina at Chapel Hill, ²New York-Presbyterian Hospital/Weill-Cornell Medical Center

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由于可用组织有限，年轻胚胎小鼠瓣膜中蛋白质表达的分析受到阻碍。本手稿提供了从发育中的胚胎小鼠瓣膜区域制备蛋白质以进行蛋白质印迹分析的方案。

该程序的总体目标是从胚胎心脏瓣膜中分离和提取蛋白质。这是通过首先从怀孕的小鼠中分离胚胎来实现的。在第二步中，将心脏从胚胎中解剖出来，然后在最后一步收集心脏瓣膜。

裂解组织并制备所得蛋白质样品用于后续分析。最终，western BLO 分析可用于评估蛋白质表达水平。这种方法的视觉演示至关重要，因为由于发育中的心脏形状小而紧凑，因此很难学习解剖步骤。

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发育生物学第91 心脏瓣膜胚胎鼠标开发蛋白质免疫印迹