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Chapters
- 00:05Title
- 02:27Electroporation of Competent Cells
- 04:35Gridding Transformants and Replica Plating to Determine the Auxotroph Phenotype
- 08:02Gene Rescue
- 11:13Results: Generation of Auxotrophic Transformants in Enterobacter sp. YSU
- 12:39Conclusion
Enterobacter sp. YSU grows in glucose minimal salts medium. Auxotrophs are generated by transforming it with a transposome which randomly inserts itself into the host genome. Mutants are found by replica plating from complex medium to minimal medium. Interrupted genes are identified by gene rescue and sequencing.
