<em>Pseudomonas aeruginosa</em> Induced Lung Injury Model

Varsha Suresh Kumar; Ruxana T. Sadikot; Jeanette E. Purcell; Asrar B. Malik; Yuru Liu

doi:10.3791/52044

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Pseudomonas aeruginosa Induced Lung Injury Model

绿脓杆菌引起的肺损伤模型

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07:24 min

October 29, 2014

DOI: 10.3791/52044-v

Varsha Suresh Kumar¹, Ruxana T. Sadikot², Jeanette E. Purcell³, Asrar B. Malik¹, Yuru Liu¹

¹Department of Pharmacology, University of Illinois College of Medicine,University of Illinois at Chicago, ²Section of Pulmonary and Critical Care Medicine, Atlanta VAMC,Emory University, ³Biologic Resources Lab,University of Illinois at Chicago

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我们开发了一种通过气管内注射细菌铜绿假单胞菌的小鼠肺损伤模型。该模型模拟肺炎期间的肺损伤，具有临床相关性。

该程序的总体目标是通过假单胞菌气原给药来模拟肺炎、小鼠的肺损伤情况。这是通过首先制备适当浓度的假单胞菌进行注射来实现的。在第二步中，小心地暴露小鼠气管，然后通过气管内注射将细菌施用到肺部。

在最后一步中，仔细监测小鼠的疾病进展。最终，可以在注射气原假单胞菌的小鼠肺中研究肺部炎症、肺泡屏障中的细胞死亡变化和肺修复。该技术的意义延伸到肺炎引起的急性肺损伤的治疗。

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