14,477 Views
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10:00 min
January 12, 2015
DOI:
10.3791/52087-v
到斑马鱼心脏发育过程中分析的心脏基因表达分布,总RNA已被提取从离体心脏。在这里,我们提出了一个协议,用于收集功能/跳动的心迅速人工清扫,从斑马鱼胚胎获得心脏特异性mRNA。
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Isolation and Characterization of Single Cells from Zebrafish Embryos
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Cite this Article
Lombardo, V. A., Otten, C., Abdelilah-Seyfried, S. Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis. J. Vis. Exp. (95), e52087, doi:10.3791/52087 (2015).
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