Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Lara Stevanato; Caroline Hicks; John D. Sinden

doi:10.3791/52410

Journal

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发育生物学

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一个人类神经干细胞系的三维文化，微小RNA的分析和推测的靶基因分化

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

JoVE 杂志
发育生物学

This content is Free Access.

JoVE 杂志发育生物学

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

一个人类神经干细胞系的三维文化，微小RNA的分析和推测的靶基因分化

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9,915 Views

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10:48 min

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April 12, 2015

DOI:

10.3791/52410-v

DOI:

10.3791/52410-v

•

10:48 min

April 12, 2015

•

4 Views

Lara Stevanato¹, Caroline Hicks¹, John D. Sinden¹

¹ReNeuron

Summary

With the intent of characterizing changes in miRNAs on differentiated human neural stem cells (hNSCs) we describe hNSC differentiation on a three dimensional system,the evaluation of changes in microRNA expression by miRNA PCR array, and computational analysis for miRNA target prediction and its validation by dual luciferase assay.