Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Angela Rossi; Antje Appelt-Menzel; Szymon Kurdyn; Heike Walles; Florian Groeber

doi:10.3791/52576

Journal

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生物工程

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三维全层皮肤的生成等效和自动伤人

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

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生物工程

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JoVE 杂志生物工程

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

三维全层皮肤的生成等效和自动伤人

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18,615 Views

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08:35 min

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February 26, 2015

DOI:

10.3791/52576-v

DOI:

10.3791/52576-v

•

08:35 min

February 26, 2015

•

34 Views

Angela Rossi*¹, Antje Appelt-Menzel*¹, Szymon Kurdyn¹, Heike Walles^1,2, Florian Groeber²

¹Department for Tissue Engineering and Regenerative Medicine,University Hospital Würzburg, ²Translational Center Würzburg, Regenerative Therapies in Oncology and Musculoskelettal Disease,Würzburg Branch of the Fraunhofer-Institute Interfacial Engineering and Biotechnology, IGB

Summary

The goal of this protocol is to build up a three-dimensional full thickness skin equivalent, which resembles natural skin. With a specifically constructed automated wounding device, precise and reproducible wounds can be generated under maintenance of sterility.