Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity

Xiaobao Li; Jisun L. Song; Alessandro Culotti; Wei Zhang; David L. Chopp; Nanxi Lu; Aaron I. Packman

doi:10.3791/52602

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity

方法表征的共同发展生物膜和生境异质性

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09:21 min

March 11, 2015

DOI: 10.3791/52602-v

Xiaobao Li¹, Jisun L. Song², Alessandro Culotti¹, Wei Zhang¹, David L. Chopp³, Nanxi Lu¹, Aaron I. Packman¹

¹Department of Civil and Environmental Engineering,Northwestern University, ²Department of Chemical and Biological Engineeering,Northwestern University, ³Department of Applied Mathematics and Engineering Sciences,Northwestern University

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生物膜与周围环境有复杂的相互作用。为了全面研究生物膜-环境相互作用，我们在这里提出了一系列方法，为生物膜发育创造异质化学环境，量化局部流速，并分析生物膜菌落内部和周围的质量传输。

该程序的总体目标是使用集成方法表征生物膜与周围环境的相互作用。构建了一个双入口微流体流动池系统，其中含葡萄糖和无葡萄糖的培养基被泵送到流动池中，产生葡萄糖梯度。将细菌添加到系统中，并允许生物膜在几天内形成。

然后通过 3D 共聚焦显微镜对生物膜进行成像，以表征溶胶运输。在生物膜内，将荧光示踪剂注入流系统，进行延时成像以表征生物膜菌落周围的流场，注入荧光微珠并进行延时成像。所得数据的溶质传输和粒子跟踪分析表明，该方法在单个设备和一组实验中评估各种化学环境下的生物膜过程的实用性。

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生物工程第97 微流体流动池化学梯度生物膜的发展粒子跟踪流量特性荧光示踪溶质运移