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瘦素受体<sup>分贝</sup> 2型糖尿病小鼠模型:胰岛分离和活细胞双光子成像完好胰岛
Lepr<sup>db</sup> Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
JoVE 杂志
医学
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JoVE 杂志 医学
Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets

瘦素受体<sup>分贝</sup> 2型糖尿病小鼠模型:胰岛分离和活细胞双光子成像完好胰岛

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9,408 Views

10:09 min

May 11, 2015

DOI:

10:09 min
May 11, 2015

9404 Views
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Summary

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We present here a protocol for the isolation of islets from the mouse model of type 2 diabetes, Leprdb and details of a live-cell assay for measurement of insulin secretion from intact islets that utilizes 2 photon microscopy.

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