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DOI: 10.3791/52715-v
Jodi R. Schilz1, K. J. Reddy2, Sreejayan Nair3, Thomas E. Johnson4, Ronald B. Tjalkens5, Kem P. Krueger3, Suzanne Clark6
1Division of Physical Therapy, Department of Orthopedics & Rehabilitation,University of New Mexico, 2Department of Ecosystem Science and Management,University of Wyoming, 3School of Pharmacy,University of Wyoming, 4Department of Environmental and Radiological Health Sciences,Colorado State University, 5Center for Environmental Medicine,Colorado State University, 6College of Pharmacy,California Northstate University
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This study investigates the treatment of production bleed water using cupric oxide nanoparticles (CuO-NPs) and assesses their cytotoxic effects on cultured human cells. The research aims to evaluate the changes in toxicity resulting from the nanoparticle treatment.
生产渗水 (PBW) 用氧化铜纳米颗粒 (CuO-NPs) 处理,并在培养的人类细胞中评估细胞毒性。该方案的目标是将天然环境样品整合到细胞培养形式中,以评估 CuO-NP 处理引起的毒性变化。
该程序的总体目标是检查氧化铜纳米颗粒处理作为生产、废水中的污染物去除过程,并使用培养的人类细胞评估其细胞毒性。这是通过首先用氧化铜纳米颗粒处理生产排放水来实现的。在处理前后评估单个元素的浓度变化。
接下来,用未经处理的生产排放水或氧化铜稀释浓缩的细胞培养基。纳米颗粒处理的生产渗水除标准细胞培养添加剂外,还制备了未经处理的生产渗出、水培养基和氧化铜、纳米颗粒处理的生产渗出。然后将水培养基应用于人肾细胞和肝细胞并培养,并在 7 天内测量活力。
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