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JoVE Journal
Environment
去除微量元素的氧化铜纳米粉体制备铀原位恢复泌水及其对细胞活力的影响
去除微量元素的氧化铜纳米粉体制备铀原位恢复泌水及其对细胞活力的影响
JoVE Journal
Environment
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JoVE Journal Environment
Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability

去除微量元素的氧化铜纳米粉体制备铀原位恢复泌水及其对细胞活力的影响

Full Text
10,317 Views
09:23 min
June 21, 2015

DOI: 10.3791/52715-v

Jodi R. Schilz1, K. J. Reddy2, Sreejayan Nair3, Thomas E. Johnson4, Ronald B. Tjalkens5, Kem P. Krueger3, Suzanne Clark6

1Division of Physical Therapy, Department of Orthopedics & Rehabilitation,University of New Mexico, 2Department of Ecosystem Science and Management,University of Wyoming, 3School of Pharmacy,University of Wyoming, 4Department of Environmental and Radiological Health Sciences,Colorado State University, 5Center for Environmental Medicine,Colorado State University, 6College of Pharmacy,California Northstate University

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Overview

This study investigates the treatment of production bleed water using cupric oxide nanoparticles (CuO-NPs) and assesses their cytotoxic effects on cultured human cells. The research aims to evaluate the changes in toxicity resulting from the nanoparticle treatment.

Key Study Components

Area of Science

  • Environmental science
  • Cell biology
  • Nanotechnology

Background

  • Production bleed water can contain contaminants that are harmful to human health.
  • Cupric oxide nanoparticles have potential applications in contaminant removal.
  • Assessing cytotoxicity is crucial for understanding the safety of treated water.
  • Cell culture models provide a controlled environment for toxicity testing.

Purpose of Study

  • To evaluate the effectiveness of CuO-NP treatment in reducing toxicity in production bleed water.
  • To assess the cytotoxic effects of treated water on human kidney and liver cells.
  • To analyze concentration changes of elements before and after treatment.

Methods Used

  • Treatment of production bleed water with CuO-NPs.
  • Preparation of test media using treated and untreated water.
  • Application of media to cultured human kidney and liver cells.
  • Measurement of cell viability over a seven-day period.

Main Results

  • Changes in elemental concentrations were observed post-treatment.
  • Cell viability was assessed to determine cytotoxic effects.
  • CuO-NP treatment showed varying effects on different cell types.
  • Results indicate potential for using CuO-NPs in water treatment applications.

Conclusions

  • Cupric oxide nanoparticles can effectively reduce toxicity in production bleed water.
  • The study highlights the importance of evaluating cytotoxicity in treated samples.
  • Further research is needed to optimize treatment processes and assess long-term effects.

Frequently Asked Questions

What are cupric oxide nanoparticles?
Cupric oxide nanoparticles are nanoscale particles of copper oxide that have potential applications in environmental remediation.
How is cytotoxicity measured in this study?
Cytotoxicity is measured by assessing the viability of human kidney and liver cells after exposure to treated media.
What is production bleed water?
Production bleed water is wastewater generated during industrial processes that may contain harmful contaminants.
Why is it important to treat production bleed water?
Treating production bleed water is essential to reduce environmental pollution and protect human health.
What are the implications of this research?
This research suggests that CuO-NPs could be a viable option for treating contaminated water, potentially improving water safety.

生产渗水 (PBW) 用氧化铜纳米颗粒 (CuO-NPs) 处理,并在培养的人类细胞中评估细胞毒性。该方案的目标是将天然环境样品整合到细胞培养形式中,以评估 CuO-NP 处理引起的毒性变化。

该程序的总体目标是检查氧化铜纳米颗粒处理作为生产、废水中的污染物去除过程,并使用培养的人类细胞评估其细胞毒性。这是通过首先用氧化铜纳米颗粒处理生产排放水来实现的。在处理前后评估单个元素的浓度变化。

接下来,用未经处理的生产排放水或氧化铜稀释浓缩的细胞培养基。纳米颗粒处理的生产渗水除标准细胞培养添加剂外,还制备了未经处理的生产渗出、水培养基和氧化铜、纳米颗粒处理的生产渗出。然后将水培养基应用于人肾细胞和肝细胞并培养,并在 7 天内测量活力。

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