Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

Sylvia T. Cheung; Soroush Shakibakho; Eva Y. So; Alice L-F Mui

doi:10.3791/52807

Journal

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生物学

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转染RAW264.7细胞用荧光素酶报告基因

Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

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生物学

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JoVE 杂志生物学

Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

转染RAW264.7细胞用荧光素酶报告基因

Please note that all translations are automatically generated. Click here for the English version.

20,530 Views

•

12:58 min

•

June 18, 2015

DOI:

10.3791/52807-v

DOI:

10.3791/52807-v

•

12:58 min

June 18, 2015

•

18 Views

Sylvia T. Cheung^1,2,3, Soroush Shakibakho^1,2, Eva Y. So^1,2, Alice L-F Mui^1,2,3

¹Immunity and Infection Research Centre,Vancouver Costal Health Research Institute, ²Department of Surgery,University of British Columbia, ³Department of Biochemistry and Molecular Biology,University of British Columbia

Summary

Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.

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转染RAW264.7细胞用荧光素酶报告基因

Summary

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