Establishment and Characterization of UTI and CAUTI in a Mouse Model

Matt S. Conover; Ana L. Flores-Mireles; Michael E. Hibbing; Karen Dodson; Scott J. Hultgren

doi:10.3791/52892

JoVE Journal
Medicine

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JoVE Journal Medicine

Establishment and Characterization of UTI and CAUTI in a Mouse Model

建立和UTI和CAUTI表征在小鼠模型

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08:40 min

June 23, 2015

DOI: 10.3791/52892-v

Matt S. Conover¹, Ana L. Flores-Mireles¹, Michael E. Hibbing¹, Karen Dodson¹, Scott J. Hultgren¹

¹Department of Molecular Microbiology and Center for Women's Infectious Disease Research,Washington University School of Medicine

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为了能够了解细菌发病机制并催生新疗法的开发，模拟尿路感染（UTI）的能力至关重要。这项工作的目标是展示实验性 UTI 和导管相关 UTI 的小鼠模型，这些模型概括和预测在人类中观察到的发现。

该程序的总体目标是展示一种可靠的方法来建立尿路感染或 UTI 和导管相关的 UTI，或在小鼠模型中建立 UTI I，并评估感染。这是通过首先正确准备细菌接种物、导管和针头来实现的。接下来，对小鼠尿路进行膀胱内注射、接种和导管插入。

然后收集尿路组织和导管以评估感染。最后，通过测定细菌组织、尿液和导管负荷和/或细胞内细菌群落或 IBC 形成来量化感染。最终，CFU 计数和松弛染色用于显示小鼠模型中尿路定植的建立持久性和严重程度。

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